Cellular reprogramming by targeting the golgi apparatus
AssigneeMUSC Foundation for Research Development
InventorsShikhar Mehrotra, Nathaniel Oberholtzer
Life of this patent
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Abstract
The present invention generally relates to methods and compositions for the generation of T cells with enhanced features and enhanced therapeutic properties. The present invention also relates to methods of treating or preventing cancer with the T cells which display enhanced features and enhanced therapeutic properties.
Claims25
A method for identifying one or more T cells with enhanced features, comprising obtaining one or more T cells, and separating from the one or more T cells, one or more T cells possessing high Golgi mass (Golgihi T cells).
A method for generating one or more T cells with enhanced features, comprising obtaining one or more T cells, and performing at least one of the following steps:
- administering to the one or more T cells to an agent that releases H2S in the one or more T cells;
- administering to the one or more T cells to an agent that over-expresses cystathione β-synthase (CBS) in the one or more T cells;
- administering to the one or more T cells to an agent that inhibits MGAT5 enzymatic activity,
- or a combination thereof.
A method of increasing the presence of Golgihi T cells in a population of T cells, the method comprising administering to the population of T cells at least one of the following:
- an agent that releases H2S in one or more T cells of the population of T cells;
- an agent that over-expresses cystathione β-synthase (CBS) in the one or more T cells in one or more T cells of the population of T cells;
- and
- an agent that inhibits MGAT5 enzymatic activity in one or more T cells of the population of T cells.
Dependencies are inferred from the claim text.