Messenger ribonucleic acids for enhancing immune responses and methods of use thereof
AssigneeModernaTX, Inc.
InventorsEric Yi-Chun HUANG, Sze-Wah TSE, Jared IACOVELLI, Kristine MCKINNEY, Kristen HOPSON
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Abstract
The disclosure features isolated mRNAs encoding a polypeptide that enhances immune responses to an antigen(s) of interest, such as polypeptides that activate Type I interferon pathway signaling or NFkB signaling, including mRNAs comprising one or more modified nucleobase. The disclosure also features methods of using the same, for example, for enhancing immune responses when administered with an antigen(s) of interest, such as to stimulate anti-cancer immune responses or anti-pathogen immune responses.
Claims31
A messenger RNA (mRNA) encoding a polypeptide that enhances an immune response to an antigen of interest in a subject, wherein the immune response comprises a cellular or humoral immune response characterized by:
- (i) stimulating Type I interferon pathway signaling;
- (ii) stimulating NFκB pathway signaling;
- (iii) stimulating an inflammatory response;
- (iv) stimulating cytokine production;
- (v) stimulating dendritic cell development, activity or mobilization;
- or
- (vi) a combination of any of (i)-(v).
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(canceled)
A composition comprising a first mRNA encoding a first polypeptide that enhances an immune response to an antigen of interest in a subject, a second mRNA encoding a second polypeptide that enhances an immune response to an antigen of interest in a subject and, optionally, a third mRNA encoding a third polypeptide that enhances an immune response to an antigen of interest in a subject, wherein the first, second and third polypeptides function downstream of at least one Toll-like receptor (TLR) to thereby enhance an immune response, and wherein the immune response comprises a cellular or humoral immune response characterized by:
- (i) stimulating Type I interferon pathway signaling;
- (ii) stimulating NFκB pathway signaling;
- (iii) stimulating an inflammatory response;
- (iv) stimulating cytokine production;
- (v) stimulating dendritic cell development, activity or mobilization;
- or
- (vi) a combination of any of (i)-(v).
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A composition comprising at least one immune potentiator mRNA, and at least one mRNA encoding an antigen of interest, wherein the immune potentiator functions downstream of at least one Toll-like receptor (TLR) to thereby enhance an immune response, and wherein the immune response comprises a cellular or humoral immune response characterized by:
- (i) stimulating Type I interferon pathway signaling;
- (ii) stimulating NFκB pathway signaling;
- (iii) stimulating an inflammatory response;
- (iv) stimulating cytokine production;
- (v) stimulating dendritic cell development, activity or mobilization;
- or
- (vi) a combination of any of (i)-(v).
-47. (canceled)
-70. (canceled)
-74. (canceled)
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-130. (canceled)
Dependencies are inferred from the claim text.