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US 2025/0170183 A1Published application
Method for enriching muse cells and obtaining exosomes, microvesicles or the secretome therefrom
AssigneeJLM ExoGrade, LLC
InventorsMichael COLGAN, Adas DARINSKAS
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Abstract
The invention relates to a method of enriching Muse cells and obtaining the exosomes, microvesicles, or the secretome therefrom, in several embodiments, the exosomes, microvesicles, secretome or Muse cells may be used in therapeutic methods or non-therapeutic methods.
Claims20
A method for preparing EVs from Muse cells comprising the steps of:
- (a) providing Muse cells;
- (b) culturing the Muse cells of (a) in a first medium for a time period sufficient to expand Muse cells in an undifferentiated state;
- (c) culturing the Muse cells of (b) in a second medium for a time period sufficient to induce production of EVs, wherein the EVs are released into the second medium;
- and
- (d) harvesting the EVs for one or more times, wherein each harvesting comprises collecting the second medium comprising the EVs to obtain a harvest.
2 more elements
A plurality of extracellular vesicles (EVs) obtained from Muse cells or a tissue comprising Muse cells.
A method for enriching Muse cells in culture comprising the steps of:
- (a) providing a mixture of cells comprising Muse cells from a tissue in a seeding medium;
- (b) culturing the cells of (a) in a expansion medium for a time period sufficient to expand Muse cells in an undifferentiated state,
- wherein Muse cells after step (b) are enriched at least by a factor of 2, 3, 4, 5, 10, 100, 103, 104, or 105 relative to the amount of Muse cells present in step (a).
Dependencies are inferred from the claim text.