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US 2025/0164490 A1Published application
Probe for universal detection of circulating tumor cells
OwnerShaun Xiaoliu Zhang, Xinping Fuinventors
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Abstract
Disclosed are probes based on papilloma virus and modified SV40 that can be used for detecting circulating tumor cells (CTCs) in the blood stream, methods for manufacturing such probes, and methods for using such probes.
Claims20
A probe for detecting circulating tumor cells (CTCs), comprising:
- a modified SV40 virus packaged into a capsid formed from the L1 and L2 capsid proteins of a human or bovine or other papillomavirus, and including a marker gene.
A process for assembling a probe that can detect circulating tumor cells (CTCs), the process comprising:
- co-transfecting papilloma virus L1 and L2 genes with a modified SV40 construct into mammalian cells, or by in vitro assembly of the modified SV40 construct into capsids formed from co-transfecting the papilloma virus L1 and L2 genes into mammalian cells.
A method for detecting circulating tumor cells (CTCs) m a patient, the method comprising:
- collecting blood from a patient;
- isolating nucleared cells from the blood;
- preparing a mixture that includes the isolated nucleared cells from the blood and a probe for detecting CTCs, wherein the probe comprises a modified SV40 virus having a modified capsid formed from the L1 and L2 capsid proteins of a papillomavirus, and containing a marker gene;
- and
- detecting CTCs in the mixture.
2 more elements
Dependencies are inferred from the claim text.