Colorimetric assay using dithiolate-grafted nanoparticles for high-throughput screening of cryoprotectants
AssigneeThe Government of the United States of America, as represented by the Secretary of the Navy
InventorsEunkeu Oh, Kimihiro Susumu, Scott A. Walper, Jozsef Czege
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Abstract
Colorimetric methods and kits for determining anti-icing effects of testing materials are presented. In embodiments, a method includes: mixing a testing material with a dithiolate-based ligand-grafted gold nanoparticle (AuNP) probe in solution, thereby generating a test sample; chilling the test sample at a predetermined temperature for a period of time; subsequent to chilling the test sample, detecting a color of the test sample; and determining anti-icing effects of the testing material based on the color of the test sample.
Claims25
A method comprising:
- mixing a testing material with a dithiolate-based ligand-grafted gold nanoparticle (AuNP) probe in solution, thereby generating a test sample;
- chilling the test sample at a predetermined temperature for a period of time;
- subsequent to chilling the test sample, detecting a color of the test sample;
- and
- determining anti-icing effects of the testing material based on the color of the test sample.
A colorimetric assay kit for determining anti-icing effects of a testing material comprising:
- a dithiolate-based ligand-grafted gold nanoparticle (AuNP) probe for mixing with test samples of a testing material in solution, wherein changes of color of the test samples after chilling indicate anti-icing effects of the testing material.
A nanoparticle probe comprising:
- a core comprising a metal;
- and
- a dithiolate-based ligand bound to the core.
A method for preparing a nanoparticle probe, the method comprising the steps of:
- providing a dithiolate-based compound;
- and mixing the dithiolate-based compound with a nanoparticle to attach the compound to the nanoparticle;
- wherein the dithiolate-based compound is:
Dependencies are inferred from the claim text.