Enhanced cytometry for tissue characterization and screening
AssigneeFive Prime Therapeutics, Inc.
InventorsNallakkan Arvindan, Stephen Mehi, Guanqing Ou
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Abstract
An optical imaging system includes a frame designed to provide mechanical coupling between a first stage and a second stage, a sample holding region located on the first stage, a lens arrangement, and a sensor array. The lens arrangement is disposed between the first stage and the second stage and is designed to receive light from a sample at the sample holding region on the first stage. The lens arrangement has a numerical aperture less than 0.1. The sensor array is coupled to the second stage and is designed to receive light passing through the lens arrangement.
Claims15
A method of detecting cell binding reactions, comprising:
- sequentially loading a plurality of probe solutions into a first channel, wherein each of the plurality of probe solutions comprises fluorescently tagged probe molecules, and wherein the plurality of probe solutions are substantially separated from one another within the first channel;
- flowing the plurality of probe solutions sequentially through the first channel and into a second channel located above a sample, such that the plurality of probe solutions contact the sample when present in the second channel;
- receiving light fluorescing from the fluorescently tagged probe molecules present at the sample using a lens arrangement disposed beneath the sample;
- and
- detecting the light fluorescing from the fluorescently tagged probe molecules at a detector disposed optically downstream from the lens arrangement.
Dependencies are inferred from the claim text.