Metal-Labeled Polymer Microbeads with Control Over Labeling Level
AssigneeThe Governing Council of the University of Toronto
InventorsMitchell A. WINNIK, Jieyi LIU
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Abstract
The present disclosure relates to metal labelled polymer microbeads and in particular to lanthanide labelled polymer microbeads for mass cytometry bead based assays and multiplexing applications. The polymer microbead comprises a copolymer comprising a structural monomer and a metal-chelating monomer.
Claims42
A metal-encoded microbead comprising:
- a copolymer comprising:
- a structural monomer, and
- a metal-chelating monomer comprising a metal and a chelator;
- wherein the chelator coordinates the metal at least at 3 sites;
- and
- wherein the structural monomer does not comprise the chelator.
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A method of preparing a metal-encoded microbead comprising
- polymerizing a structural monomer in the presence of a steric stabilizer in a nucleation stage to obtain a first mixture comprising polymerized structural monomer, unpolymerized structural monomer, and the steric stabilizer;
- combining the first mixture with a metal-chelating monomer comprising a metal and a chelator attached to at least one polymerizable end group to obtain a second mixture,
- wherein the chelator coordinates the metal at least at 3 sites and wherein the metal-chelating monomer is polymerizable with the structural monomer;
- and
- polymerizing the second mixture to form a copolymer of the microbead;
- wherein the structural monomer does not comprise the chelator.
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Dependencies are inferred from the claim text.