# US 2025/0155431 A1 · Macroscopic laser-induced graphene > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2025/0155431 A1 - Document ID: US20250155431A1 - Status: Published application - Kind: A1 - Application number: 18/726,937 - Filed: 2023-01-06 - Published: 2025-05-15 - Earliest claimed: 2022-01-06 - Assignees: B.G. Negev Technologies and Applications Ltd., At Ben-Gurion University - Applicants: B.G. Negev Technologies and Applications Ltd., At Ben-Gurion University - Inventors: Christopher ARNUSCH; Assaf YA’AKOBOVITZ; Chetan Prakash SHARMA; Yahav BEN-SHIMON - Web page: https://patentgrep.com/patent/US20250155431A1 ## CPC - G01N 33/54373: {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings} - C01B 32/184: Preparation - G01N 29/12: by measuring frequency or resonance of acoustic waves {(measuring frequency or resonant frequency of mechanical vibrations or acoustic waves in general G01H1/06, G01H3/04, G01H13/00; acoustic resonators G10K11/04; vibration or shock testing of structures G01M7/00)} - G01N 33/56983: {Viruses} - B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites - C01B 2204/04: Specific amount of layers or specific thickness - C01B 2204/32: Size or surface area - C01P 2002/82: by IR- or Raman-data - C01P 2004/03: obtained by SEM - C01P 2004/20: extending in two dimensions, e.g. plate-like - G01N 2291/014: Resonance or resonant frequency - G01N 2333/165: Coronaviridae, e.g. avian infectious bronchitis virus - G01N 2469/10: Detection of antigens from microorganism in sample from host - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C01B: NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; {METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C} - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES - C01P: INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS ## Abstract Provided herein macroscopic laser-induced graphene flakes and sheets, methods of manufacturing same, and the use of same in diagnostic and other applications. ## Claims ### Claim 1 A standalone macroscopic graphene foam sheet flake, wherein said graphene foam is laser-induced graphene, wherein said standalone sheet flake is not supported on a carrier or embedded in a polymer, wherein said standalone sheet flake is mechanically integral, and wherein said flake has a thickness of between 5 and 200 microns. ### Claim 2 (depends on 1) The flake according to claim 1, characterized by a porous stochastic morphology on one side, and a honeycomb-like cellular structure of a plurality of interconnected cells having a plan projection of a symmetric or asymmetric polygon having between 3 and 10 sides on the opposing side, as seen under suitable magnification using scanning electron microscopy (SEM). ### Claim 3 (depends on 2) The flake according to claim 2, wherein said honeycomb-like cellular structure has an overall appearance of co-oriented fused laser-induced graphene continuous-flake fibers. ### Claim 4 (depends on 2) The flake according to claim 2, wherein said honeycomb-like cellular structure has an overall appearance of an essentially uniform worm-like patterned laser-induced graphene continuous flake sheet with randomly oriented interfused laser-induced graphene continuous-flake fibers. ### Claim 5 (depends on 1) The flake according to claim 1, wherein said flake has a thickness of between 20 and 40 microns. ### Claim 6 (depends on 1) The flake according to claim 1, wherein said macroscopic flake has a length of at least 1 millimeter. ### Claim 7 (depends on 6) The flake according claim 6, wherein said length of at least 10 millimeters. ### Claim 8 (depends on 1) The flake according to claim 1, wherein said flake is mechanically integral over an area of between 1 square centimeter and 1,000 square centimeters. ### Claim 9 (depends on 1) The flake according to claim 1, further comprising a targeting moiety associated therewith. ### Claim 10 (depends on 7) The flake according to claim 7, wherein said targeting moiety is an anti-SARS-CoV-2 spike receptor-binding domain antibody. ### Claim 11 (depends on 1) A resonating sensor comprising a standalone macroscopic graphene foam sheet flake according to claim 1. ### Claim 12 (depends on 1) Method of manufacturing of standalone macroscopic graphene foam sheet flakes as defined in claim 1, comprising providing a sheet of a polymer, and irradiating said sheet of polymer with laser having power or energy density equivalent to that obtainable with laser power output of 4.5 to 9 W from a laser equipped with a lens providing a pulse diameter of 130 microns at 1000 pulses per inch density, wherein a focal offset of said laser is between about 100.2% and 101.2% of the focal length of said laser lens, or between 98% and 90% of the focal length of said lens, and wherein a minimum irradiation area of said polymer between 1 and 4 square centimeters. ### Claim 13 (depends on 12) The method according to claim 12, wherein said polymer is a polyimide. ### Claim 14 (depends on 12) The method according to claim 12, wherein said irradiation area is between 1 square centimeter and 1 square meter. ### Claim 15 (depends on 14) The method according to claim 14, wherein said focal offset of said laser is 90 and 98% of said focal length of said lens, and wherein said irradiation area is between 40 and 200 square centimeters.