Methods and Compositions for Evaluating Biomarkers in Salivary Exosomes and Evaluating Cognitive Fatigue
AssigneesThe Regents of the University of California, The United States Government as represented by the Department of Veterans Affairs
InventorsVarghese John, Bruce Teter, Samuel Whitaker Johnson-Cohn
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Abstract
Disclosed herein are methods and compositions for diagnosing cognitive fatigue. Specifically, the disclosure provides a method of diagnosing a subject as suffering from cognitive fatigue, which comprises measuring the amount of a biomarker, comprising phosphoglycerate kinase 1 (PGK1) and/or miR3185, in an exosome sample obtained from a saliva sample from the subject, comparing the amount with a control, and identifying the subject as suffering from cognitive fatigue.
Claims19
A method of preparing an exosome sample for measuring the amount of a biomarker, preferably PGK1 and/or miR3185, therein, which comprises obtaining a saliva sample from one or more subjects, adding a protease inhibitor to the saliva sample, removing solids in the saliva sample to obtain a supernatant, and isolating exosomes present in the supernatant on a substrate surface.
(canceled)
A method of diagnosing a subject as suffering from cognitive fatigue, which comprises measuring the amount of a biomarker, which is PGK1 and/or miR3185, in an exosome sample obtained from a saliva sample from the subject, comparing the amount with a control, and identifying the subject as suffering from cognitive fatigue where the measured amount of PGK1 is about 1.3-1.4 fold increase and/or the amount of miR3185 is about 1.2-1.3 decrease compared to the control.
A method of evaluating expression levels of PGK1 and miR3185 in a subject, which consists of:
- obtaining an exosome sample from a saliva sample from the subject,
- measuring the amount of the biomarker in the exosome sample, and
- optionally measuring in the exosome sample the amount of one or more additional biomarkers selected from the group consisting of:
- LEG1, AMY1A, BPIFA2, CA6, DPP4, DMBT1, hsa-miR-518e-3p, hsa-miR-182-5p, hsa-miR-614, hsa-miR-1296-3p, hsa-miR-126-3p, hsa-miR-1257, hsa-miR-134-3p, hsa-miR-105-5p, hsa-miR-4536-5p, hsa-miR-642a-5p, and hsa-miR-140-5p.
Dependencies are inferred from the claim text.