Flexible substrates for nucleic acid synthesis
AssigneeTwist Bioscience Corporation
InventorBill James Peck
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Abstract
Provided herein are compositions, devices, systems and methods for the generation and use of biomolecule-based information for storage. Further described herein are highly efficient methods for long term data storage with 100% accuracy in the retention of information. Additionally, devices described herein for de novo synthesis of oligonucleic acids encoding information related to the original source information may have a flexible material for oligonucleic acids extension.
Claims22
1-16. (canceled)
A method for storing information, the method comprising:
- a) converting an item of information in the form of at least one digital sequence to at least one nucleic acid sequence;
- b) synthesizing a plurality of oligonucleic acids having predetermined sequences collectively encoding for the at least one nucleic acid sequence, wherein the plurality of oligonucleic acids comprises at least about 10,000 oligonucleic acids, wherein the plurality of oligonucleic acids collectively encode for a sequence that differs from the predetermined sequences by no more than 1 base in 1000, and wherein each oligonucleic acid of the plurality of oligonucleic acids comprises from 50 to 500 bases in length;
- and
- c) storing the plurality of oligonucleic acids.
18-21. (canceled)
24-29. (canceled)
A method for storing information, the method comprising:
- a) encrypting at least one item of information in the form of at least one digital sequence to at least one nucleic acid sequence;
- b) synthesizing a plurality of oligonucleic acids having predetermined sequences collectively encoding for the at least one nucleic acid sequence, wherein the plurality of oligonucleic acids comprises at least about 10,000 oligonucleic acids, and wherein each oligonucleic acid of the plurality of oligonucleic acids comprises from 50 to 500 bases in length;
- c) storing the plurality of oligonucleic acids;
- d) sequencing the plurality of oligonucleic acids;
- e) decrypting the plurality of oligonucleic acids from a nucleic acid sequence to a digital sequence;
- and
- f) assembling the digital sequence to form the at least one digital sequence, wherein the at least one digital sequence is recovered with 100% accuracy.
A device for information storage, the device comprising:
- a) a flexible structure having a surface;
- and
- b) a plurality of features on the surface, wherein each feature has a width of from about 1 to about 500 μm, and wherein each feature of the plurality of features is coated with a moiety that binds to the surface and comprises a hydroxyl group available for nucleoside coupling.
Dependencies are inferred from the claim text.