Reduced pantothenic acid levels in fermentative production of oligosaccharides
AssigneeChr. Hansen A/S
InventorsHenning FRERIGMANN, Katja PARSCHAT, Markus ENGLERT, Christian TROETSCHEL
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Abstract
Disclosed are means and methods for the fermentative production of an oligosaccharide of interest by a genetically engineered microbial cell, wherein concomitant biosynthesis of pantothenic acid is reduced or abolished.
Claims14
A genetically engineered microbial cell for the production of an oligosaccharide of interest, wherein said microbial cell possess a metabolic pathway for the intracellular biosynthesis of the oligosaccharide of interest, and a low level of or no intracellular biosynthesis of pantothenic acid.
(canceled)
A method for producing an oligosaccharide of interest, the method comprises
- providing a genetically engineered microbial cell which possesses a metabolic pathway for the intracellular biosynthesis of the oligosaccharide of interest, and a low level of or no intracellular biosynthesis of pantothenic acid;
- culturing the genetically engineered microbial cell in a medium and under conditions that are permissive for the intracellular biosynthesis of the oligosaccharide of interest;
- and
- optionally retrieving the oligosaccharide of interest.
(canceled)
Dependencies are inferred from the claim text.