Tomatoes containing high levels of 7-dehydrocholesterol and preparation method therefor
AssigneeGflas Life Sciences, Inc.
InventorsSunghwa CHOE, Sunmee CHOI, Jinhwa KIM, Jeongmo KIM, Min Kyoung YOU, Yun-A JEON
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Abstract
The present invention relates to a tomato containing a high concentration of a precursor of vitamin D3, produced by using gene-editing technology, and a method for producing the same. Specifically, it has been confirmed that a tomato having the DWF5-1 gene deleted accumulate a high concentration of 7-dehydrocholesterol and that seeds can be produced therefrom. Thus, a tomato produced in such a way can contain a high concentration of a precursor of vitamin D3 and have a high commercial applicability.
Claims24
A transformed tomato containing a high concentration of 7-dehydrocholesterol of Formula 1 below.
A vector comprising at least one sgRNA (single guide RNA) that complementarily binds to a nucleotide sequence of the DWF5-1 gene and a nucleotide sequence encoding a CRISPR (clustered regularly interspaced palindromic repeats)-associated protein.
Dependencies are inferred from the claim text.