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US 2025/0154475 A1Published application
Mutant ketoreductase with increased ketoreductase activity as well as methods and uses involving the same
AssigneeHoffmann-La Roche Inc.
InventorsHans Iding, Irene Marzuoli
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Abstract
The present invention relates to a mutant ketoreductase, a nucleic acid encoding the mutant ketoreductase, a vector comprising the nucleic acid, a method for the enzymatic reduction of a prochiral ketone and the formation of a chiral alcohol with the mutant ketoreductase, the use of the mutant ketoreductase for the preparation of chiral alcohols as well as the use of the method for the preparation of pharmaceutically active morpholine compounds.
Claims19
A mutant ketoreductase with increased ketoreductase activity relative to the wild-type ketoreductase,
- wherein the mutant ketoreductase comprises an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 1 (Lactobacillus brevis ATCC 14869 ketoreductase);
- and
- wherein the mutant ketoreductase has at least two amino acid substitutions relative to the amino acid sequence of SEQ ID NO:
- 1, wherein
- the amino acid at the position corresponding to position 145 of SEQ ID NO:
- 1 is substituted with Leu, Ala, Cys, Met or Thr (Leu145, Ala145, Cys145, Met145 or Thr145, respectively) and
- the amino acid at the position corresponding to position 202 of SEQ ID NO:
- 1 is substituted with Cys, Glu, Ile, Leu or Thr (Cys202, Glu202, Ile202, Leu202 or Thr202, respectively).
6 more elements
Dependencies are inferred from the claim text.