Human-induced pluripotent stem cell overexpressing tlx and use thereof

A transcription unit, comprising a CMV promoter or an EFIA promoter, and a nucleic acid molecule the nucleic acid molecule comprising:

• a nucleic acid sequence set forth in SEQ ID NO:
• 3 that encodes a protein set forth in SEQ ID NO:
• 1, and or
• a nucleic acid sequence set forth in SEQ ID NO:
• 4 that encodes otein set forth in SEQ ID NO:
• 2.

A method for producing an exosome of neural stem cells (NSCs), comprising:

• using a TLX for constructing human induced pluripotent stem cells (hiPSCs) that self-drivingly differentiate into neural stem cells (NSCs), the TLX comprising at least one selected from the group consisting of the following I to V:
• I), a TLX protein having an animno acid sequence set forth in SEQ. ID. NO:
• 1,
• II), a protein having an amino acid sequence of the TLX protein in I) by substitution, deletion or addition of one or more amino acids and with a function identical or similar to the TLX protein,
• III), a nucleic acid molecule encoding the protein in I) or II), and
• IV), a nucleic acid molecule having a nucleotide sequence of the nucleic acid molecule in III) by substitution, deletion or addition of one or more nucleotides and encoding a protein with an identical or similar function;
• and
• V), an substance regulating a level or activity of at least one selected from the group consisting of I) to IV);
• culturing the hiPSCs by using a culture medium to obtain an immortalized NSC, wherein the culture medium comprises a basal culture medium and one selected from the group consisting of BSA, Glowamax additive, sodium pyruvate, NaCl, N2 trophic factor, B27 neurotrophic factor, insulin, nonessential amino acid, FGF2, EGF, heparin and a combination thereof;
• culturing the immortalized NSC;
• collecting a supernatant;
• and
• extracting an exosome of NSCs.