Methods and Applications of Analyzing the Perfusate of an Ex Situ Perfused Kidney
AssigneesVib Vzw, Katholieke Universiteit Leuven
InventorsBart Ghesquière, Ina Jochmans
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Abstract
The invention relates to the field of analyzing parameters in the perfusate of an organ under ex situ organ perfusion conditions. In particular the identified parameters include 1 or more of arginine, glutamate, glucose, glutamine, lactate, alanine, leucine and isoleucine; and are capable of accurately determining the extent of damage present in the perfused organ. Several applications of the assessment of these identified parameters are also part of the invention.
Claims9
A method of analyzing the perfusate of a kidney which is ex vivo or ex situ perfused, comprising:
- sampling the perfusate during or after initiating the perfusion;
- determining the presence, level or concentration of the analyte arginine in the perfusate sample(s).
A method of therapeutic optimization of, repair of, or reconditioning of a kidney prior to transplantation, comprising one or more steps of:
- connecting the kidney to an ex vivo or ex situ perfusion device;
- performing the therapeutic optimization, repair or reconditioning of the perfused kidney during perfusion;
- sampling the perfusate during or after initiating the perfusion;
- determining the presence, level or concentration of the analyte arginine in the perfusate sample(s).
A method of developing or optimizing kidney perfusion solutions or kidney preservation solutions, or of optimizing kidney perfusion conditions, comprising:
- connecting the kidney to an ex vivo or ex situ perfusion device;
- perfusing the kidney with a test perfusion or preservation solution, or perfusing the kidney under test perfusion conditions;
- monitoring the presence, level or concentration of the analyte arginine in the perfusate.
A method of screening for modalities capable of reversing or partially reversing defects of an kidney;
- or of screening for modalities capable of reversing or partially reversing ischemic damage in a kidney;
- or of screening for modalities capable of improving or enhancing kidney function, comprising one or more steps of:
- connecting the kidney to an ex vivo or ex situ perfusion device;
- adding to the perfusion solution a test modality, wherein the test modality is designed to reverse or to partially reverse a defect of or in a kidney;
- or wherein the test modality is designed to reverse or to partially reverse ischemic damage in a kidney;
- or wherein the test modality is designed to improve or enhance kidney function;
- monitoring the presence, level or concentration of the analyte arginine in the perfusate.
The method according to any of the foregoing claims further comprising determining the presence, level or concentration one or more of the analytes glutamate, glutamine, glucose, lactate, alanine, leucine and/or isoleucine in the perfusate sample(s);
A machine perfusion apparatus or device, an organ transporter, or an organ cassette, further characterized in that it is comprising a unit or module for determining the presence, level or concentration of the analyte arginine in the perfusate of an organ ex situ perfused with a perfusion solution.
A method of analyzing the perfusate of an organ perfused ex vivo or ex situ with a perfusion solution, comprising:
- sampling the perfusate at different time points during the ex vivo or ex situ perfusion;
- determining a reference time point which is the time point during perfusion at which the organ wash-out fraction has homogenized with the initial perfusion solution;
- and, optionally,
- setting the presence, level or concentration of an analyte of interest determined in the perfusate at the reference time point as reference or control level or concentration of the analyte of interest.
Dependencies are inferred from the claim text.