Biotin-trap beads and methods of production and use thereof

A method of producing a biotin-trap composition, the method comprising the steps of:

• (1) obtaining a particle having an outer surface with functional groups thereon;
• (2) reacting the particle with dextran aldehyde polymer under conditions that form an inner dextran aldehyde polymer coating layer disposed on at least a portion of the outer surface of the particle;
• (3) reacting the particle with a biotin-specific binding partner under conditions that conjugate the biotin-specific binding partner to the inner dextran aldehyde polymer coating layer, wherein the biotin-specific binding partner is avidin or an analog thereof;
• (4) reacting the particle with tetraethylene pentaamine (TPA) or poly(ethylene amine) n (where n is 1 to 20);
• and
• (5) reacting the particle with dextran aldehyde polymer under conditions that form an outer dextran aldehyde polymer coating layer.