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US 2025/0147009 A1Published application
Methods and compositions for removing biotin interference from assays using molecular traps
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsTie Wei, Zhu Teng, Izak Bahar
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Abstract
The present disclosure relates to methods and compositions to remove or reduce biotin interference from certain assays.
Claims20
A method of reducing interference by free-hapten in an assay for detection of a target analyte in a patient sample, the method comprising:
- combining the patient sample, at least two assay components for detection of the target analyte, and a trap to form an assay solution, wherein at least one of the at least two assay components contains an analyte-specific binding partner (sbp), and wherein a first assay component of the at least two assay components comprises a hapten conjugate and a second assay component of the at least two assay components comprises a hapten-specific binding partner that binds to the hapten conjugate of the first assay component, and wherein the trap is selective for the free-hapten;
- and
- selectively retaining the free-hapten in the assay solution with the trap;
- and
- wherein the trap comprises a molecular structure that selectively binds to free-hapten, wherein the molecular structure is soluble in an assay solution, the molecular structure comprising:
- a modified specific binding partner (sbp) to the free-hapten, wherein the modified sbp to free-biotin is selected from a modified avidin, a modified streptavidin, or a modified traptavidin, and wherein the modified sbp to free-fluorescein comprises a modified anti-fluorescein, wherein the modified sbp is modified by the attachment of one or more of dextran aldehyde, amino dextran, or bovine serum albumin thereto, and wherein the modification to the sbp prevents the hapten-conjugate detected by the assay from binding to the modified sbp;
- and
- a coating comprising a hydrophobic moiety.
5 more elements
A method of reducing interference by free-hapten in an assay for detection of a target analyte in a patient sample, the method comprising:
- combining the patient sample, at least two assay components for detection of the target analyte, and a trap to form an assay solution, wherein at least one of the at least two assay components contains an analyte-specific binding partner (sbp), and wherein a first assay component of the at least two assay components comprises a hapten conjugate and a second assay component of the at least two assay components comprises a hapten-specific binding partner that binds to the hapten conjugate of the first assay component, and wherein the trap is selective for the free-hapten;
- and
- selectively retaining the free-hapten in the assay solution with the trap;
- and
- wherein the trap comprises a molecular structure that selectively binds to free-hapten, wherein the molecular structure is soluble in an assay solution, the molecular structure comprising:
- a modified specific binding partner (sbp) to the free-hapten, wherein the modified sbp to free-biotin is selected from a modified avidin, a modified streptavidin, or a modified traptavidin, and wherein the modified sbp to free-fluorescein comprises a modified anti-fluorescein, wherein the modified sbp is modified by the attachment of one or more of dextran aldehyde, amino dextran, or bovine serum albumin thereto, and wherein the modification to the sbp prevents the hapten-conjugate detected by the assay from binding to the modified sbp;
- and
- a coating comprising a hydrophobic moiety, wherein the hydrophobic moiety of the coating of the trap is sulfo-N-hydroxy succinimide acetate.
5 more elements
A method of reducing interference by free-hapten in an assay for detection of a target analyte in a patient sample, wherein the free-hapten is free-biotin or free-fluorescein, the method comprising:
- combining the patient sample, at least two assay components for detection of the target analyte, and a trap to form an assay solution, wherein at least one of the at least two assay components contains an analyte-specific binding partner (sbp), and wherein a first assay component of the at least two assay components comprises a hapten conjugate and a second assay component of the at least two assay components comprises a hapten-specific binding partner that binds to the hapten conjugate of the first assay component, and wherein the trap is selective for the free-hapten;
- and
- selectively retaining the free-hapten in the assay solution with the trap;
- and
- wherein the trap comprises a molecular structure that selectively binds to free-hapten, wherein the molecular structure is soluble in an assay solution, the molecular structure comprising:
- a modified specific binding partner (sbp) to the free-hapten, wherein the modified sbp to free-biotin is selected from a modified avidin, a modified streptavidin, or a modified traptavidin, and wherein the modified sbp to free-fluorescein comprises a modified anti-fluorescein, wherein the modified sbp is modified by the attachment of one or more of dextran aldehyde, amino dextran, or bovine serum albumin thereto, and wherein the modification to the sbp prevents the hapten-conjugate detected by the assay from binding to the modified sbp;
- and
- a coating comprising a hydrophobic moiety, wherein the hydrophobic moiety of the coating of the trap is sulfo-N-hydroxy succinimide acetate.
5 more elements
Dependencies are inferred from the claim text.