US 2025/0146987 A1 · FIRST-DIMENSION-GUIDED DIFFERENTIAL RESCALING OF CONTOUR PLOT IN COMPREHENSIVE TWO-DIMENSIONAL GAS CHROMATOGRAPHY

Abstract

A first-dimension-guided differential scaling algorithm of the contour plot in comprehensive two-dimensional gas chromatography (2D GC) system is developed by incorporating both 1D and 2D chromatograms obtained by first and second detectors. This differential scaling method is shown to significantly improve 2D GC results in terms of retention time accuracy and consistency, peak width and hence peak capacity, and analyte quantification accuracy, which are inevitably affected by the modulation period and phase shift in modulation when the conventional contour plot reconstruction methods. Furthermore, the differential scaling method is shown to better handle the coelution and missing peak issues often encountered in the conventional methods. Finally, the differential scaling method exhibits high versatility in detector selection, which greatly broadens the 2D GC utility and can be easily adapted to other 2D chromatography systems.

Claims17

Dependent claims

A method for producing a two dimensional chromatogram, comprising:

measuring, by a first detector, eluent exiting a first column of a chromatography system to form first eluent data;
measuring, by a second detector, eluent exiting a second column of the chromatography system to form second eluent data, where the second column is downstream from the first column;
modulating the eluent passing from the first column to the second column at a modulation frequency;
partitioning the second eluent data into a plurality of data slices separated at time intervals according to the modulation frequency;
scaling the second eluent data to form scaled second eluent data, where the second eluent data is scaled in part using the first eluent data;
interpolating additional eluent data from the scaled second eluent data, where the additional eluent data is generated at time intervals less than time intervals specified by the modulation frequency;
scaling the additional eluent data to form scaled additional eluent data, where the additional eluent data is scaled in part using the first eluent data;
and
generating a two dimensional chromatogram from the scaled second eluent data and the scaled additional eluent data.

6 more elements

A chromatography system for producing a two dimensional chromatogram, comprising:

an injection port configured to receive an eluent;
a first separation column fluidly coupled to the injection port;
a first detector fluidly coupled to an outlet of the first separation column and configured to measure eluent exiting the first separation column to form first eluent data;
a second separation column fluidly coupled to and positioned downstream from the first detector;
a second detector fluidly coupled to an outlet of the second separation column and configured to measure eluent exiting the second separation column to form second eluent data;
a modulator fluidly coupled between an outlet of the first detector and an inlet of the second separation column and configured to modulate the eluent passing from the first separation column to the second separation column at a modulation frequency;
and
a controller interfaced with the first detector, the second detector and the modulator.

5 more elements

A method for producing a two dimensional chromatogram, comprising:

measuring, by a first detector, eluent exiting a first column of a chromatography system to form first eluent data;
measuring, by a second detector, eluent exiting a second column of the chromatography system to form second eluent data, where the second column is downstream from the first column;
modulating the eluent passing from the first column to the second column at a modulation frequency;
partitioning the second eluent data into a plurality of data slices separated at time intervals according to the modulation frequency;
rescaling the second eluent data in accordance with the first eluent data;
and
generating a two dimensional chromatogram from the rescaled second eluent data.

4 more elements

Dependencies are inferred from the claim text.

First-dimension-guided differential rescaling of contour plot in comprehensive two-dimensional gas chromatography

Life of this patent

First-dimension-guided differential rescaling of contour plot in comprehensive two-dimensional gas chromatography

Life of this patent

Abstract

Claims17