# US 2025/0146938 A1 · Systems for nanoplastics detection and method of using the same

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- Number: US 2025/0146938 A1
- Document ID: US20250146938A1
- Status: Published application
- Kind: A1
- Application number: 18/931,616
- Filed: 2024-10-30
- Published: 2025-05-08
- Earliest claimed: 2023-11-03
- Inventors: Vidhatri L. Iyer
- Web page: https://patentgrep.com/patent/US20250146938A1

## CPC

- G01N 21/643: {non-biological material}
- G01N 1/4077: {by other techniques involving separation of suspended solids}
- G01N 33/18: Water
- G01N 33/1813: {Specific cations in water, e.g. heavy metals}
- G01N 33/1826: {Organic contamination in water}
- G01N 2001/4088: {filtration}
- G01N 2021/6439: {with indicators, stains, dyes, tags, labels, marks}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method of detecting nanoplastics in water includes collecting a water sample from the water. The method includes filtering the water sample to remove debris from the water sample to form a filtered water sample. The method includes adding a lipophilic fluorescent dye to the filtered water sample to form a mixture. The method includes incubating the mixture for an incubation time period at a predetermined temperature. The method includes measuring a nanoplastics concentration in the mixture using a measurement device at a predetermined wavelength.

## Claims

### Claim 1

A method of detecting nanoplastics in water, the method comprising:
providing a 0.45 μm syringe and a 0.45 μm syringe filter, collecting a water sample from the water, wherein the water is wastewater,
filtering the water sample with the 0.45 μm syringe and the 0.45 μm syringe filter to remove debris from the water sample to form a filtered water sample,
adding a lipophilic fluorescent dye to the filtered water sample to form a mixture,
incubating the mixture for an incubation time period at a predetermined temperature, wherein the incubation time period is less than 20 minutes, and
measuring a nanoplastics concentration in the mixture using a measurement device at a predetermined wavelength.

### Claim 2

(canceled)

### Claim 3 (depends on 1)

The method of claim 1, wherein the lipophilic fluorescent dye comprises Nile Red dye.

### Claim 4 (depends on 1)

The method of claim 1, wherein the mixture comprises about 20% methanol concentration.

### Claim 5 (depends on 1)

The method of claim 1, wherein the incubation time period is about 10 minutes.

### Claim 6 (depends on 1)

The method of claim 1, wherein the predetermined wavelength is about 450 nanometers.

### Claim 7 (depends on 1)

The method of claim 1, wherein the mixture is not agitated.

### Claim 8 (depends on 1)

The method of claim 1, wherein the predetermined temperature is room temperature.

### Claim 9 (depends on 1)

The method of claim 1, wherein the 0.45 μm syringe filter comprises hydrophobic polytetrafluoroethylene (PTFE) fluorophore.

### Claim 10 (depends on 1)

The method of claim 1, further comprising providing a plurality of Eppendorf tubes.

### Claim 11 (depends on 10)

The method of claim 10, further comprising providing a plurality of 1 mL tuberculin syringes.

### Claim 12 (depends on 1)

The method of claim 1, further comprising positioning the mixture on an orbital shaker and agitating the mixture with the orbital shaker.

### Claim 13 (depends on 1)

The method of claim 1, further comprising powering the measurement device with a 5V DC power adaptor or a plurality of batteries.