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US 2025/0146181 A1Published application
Methods and systems for droplet-based single cell barcoding
Assignee10x Genomics, Inc.
InventorsZahra Kamila Belhocine, Rajiv Bharadwaj, Christopher Hindson, Michael Schnall-Levin, Bill Kengli Lin, Anthony Makarewicz, Pranav Patel, Katherine Pfeiffer, Andrew D. Price, Mohammad Rahimi Lenji, Tobias Daniel Wheeler, Yifeng Yin
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Abstract
Methods and systems are provided for sample preparation techniques and sequencing of macromolecular constituents of cells and other biological materials.
Claims20
A method of analyzing chromatin, comprising:
- (a) providing a mixture comprising (i) a biological particle comprising (1) chromatin comprising a template deoxyribonucleic acid (DNA) and (2) a protein, and (ii) a plurality of nucleic acid barcode molecules;
- (b) contacting said biological particle with a labelling agent comprising a reporter oligonucleotide, wherein said labelling agent is configured to couple to said protein;
- (c) generating a plurality of template DNA fragments of said chromatin using a plurality of transposase complexes;
- (d) generating a first barcoded nucleic acid molecule using (i) a template DNA fragment of said plurality of template DNA fragments and (ii) a first nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules;
- and
- (e) generating a second barcoded nucleic acid molecule using (i) said reporter oligonucleotide and (ii) a second nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules.
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Dependencies are inferred from the claim text.