Methods for generating functional self-replicating rna molecules
AssigneeReplicate Bioscience, Inc.
InventorsNathaniel Stephen Wang, Shigeki Joseph MIYAKE-STONER
Life of this patent
Estimating the term…
Abstract
The present disclosure relates generally to methods of generating functional self-replicating RNAs from non-functional positive-sense single-stranded RNA (+ssRNA) virus genomes such as alphavirus genomes or srRNAs. More particularly, the methods pertain to the assembly of a plurality of nucleic acid fragments to generate de novo functional srRNAs. Also provided are compositions, nucleic acid constructs, vectors and recombinant cells including such functional srRNAs. Further disclosed herein are methods for inducing a pharmacodynamic effect in a subject as well as methods for preventing and/or treating a health condition in a subject in need thereof.
Claims66
A method for generating a functional self-replicating RNA (srRNA), the method comprising:
- (a) providing one or more positive-sense single-stranded RNA (+ssRNA) viral genomes or non-functional srRNAs, wherein at least one of the one or more +ssRNA viral genomes or srRNAs is non-functional;
- (b) removing one or more RNA polymerase termination sites or an RNA polymerase cryptic termination sites from the one or more +ssRNA viral genomes or srRNAs;
- (c) generating a plurality of nucleic acid fragments each comprising a nucleotide sequence derived from the one or more +ssRNA viral genomes or srRNAs;
- and
- (d) assembling the plurality of nucleic acid fragments to generate a de novo functional srRNA assembly.
A method for preventing or treating a health condition in a subject, the method comprises administering to the subject a composition comprising:
- (a) a functional srRNA of claim 350;
- (b) a nucleic acid construct of claim 36;
- (c) a recombinant cell of any one of claims 38 to 45;
- and/or
- (d) a pharmaceutical composition of any one of claims 46 to 56.
Dependencies are inferred from the claim text.