Hyaluronic acid-producing recombinant cells
AssigneeGivaudan SA
InventorsDominique LOUIS, Valentina BEVILACQUA, Lionel DURANT, Karine JAILLARDON, Dominique THOMAS
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Abstract
The present invention relates to the field of bio-production of hyaluronic acid. There is a need in the art for hyaluronic acid production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a genetically modified cell comprising many modifications as described in the present text. The present invention further proposes methods allowing the bio-production of hyaluronic acid having a controlled molecular weight using the genetically modified cells of the invention.
Claims31
A recombinant host cell (i) or a recombinant yeast cell ii) producing hyaluronic acid (HA) wherein the recombinant host cell (i) or the recombinant yeast cell ii) comprises:
- (a) one or more recombinant nucleic acids encoding a polypeptide having hyaluronan synthase activity;
- (b) one or more recombinant nucleic acids encoding a polypeptide having UDP-Glucose dehydrogenase (UDP-GlcDH or HASB) activity;
- (c) one or more recombinant nucleic acids encoding a polypeptide having hyaluronidase activity wherein the polypeptide having hyaluronidase activity comprises a secretion signal so that hyaluronic acid is produced by the recombinant host cell (i) or the recombinant yeast cell ii), and wherein the hyaluronic acid produced by the recombinant host cell (i) is of a desired molecular weight (HAMW);
- (d) (i) one or more recombinant nucleic acids encoding a polypeptide having a glutamine synthetase (GLN1) activity;
- and/or
- (iie) one or more disrupted endogeneous nucleic acids encoding a glutamate synthase (GLT1);
- wherein said recombinant yeast cell ii) belongs to a genus selected from the group consisting of Saccharomyces, Candida, Kluyveromyces, Ogataea, Yarrowia, Debaryomyces and Ashbya.
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Dependencies are inferred from the claim text.