Method for assessing differentiation potential of cells in culture broth in differentiation of pluripotent stem cells into neural cells of midbrain floor plate region

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising:

• measuring a concentration of NT-3 in each of a first culture supernatant and a second culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region;
• determining a rate of change in the concentration of NT-3 from the concentration of NT-3 in the first culture supernatant and the concentration of NT-3 in the second culture supernatant;
• comparing an absolute value of the rate of change in the concentration of NT-3 with a reference rate of change;
• and
• when the absolute value of the rate of change in the concentration of NT-3 is equal to or more than the reference rate of change, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein
• the first culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 192 hours after the start of culture of the pluripotent stem cells, and
• the second culture supernatant is a culture supernatant collected from the culture broth at any time from 24 hours to 96 hours before collection of the first culture supernatant or at any time from 24 hours to 96 hours after collection of the first culture supernatant.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising:

• measuring a concentration of NT-3 in each of a first culture supernatant, a second culture supernatant, and a third culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region;
• comparing the concentration of NT-3 in the first culture supernatant, the concentration of NT-3 in the second culture supernatant, and the concentration of NT-3 in the third culture supernatant;
• and
• when the concentration of NT-3 in the first culture supernatant is higher than the concentration of NT-3 in the second culture supernatant and the concentration of NT-3 in the third culture supernatant, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein
• the first culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 192 hours after the start of culture of the pluripotent stem cells,
• the second culture supernatant is a culture supernatant collected from the culture broth at any time from 24 hours to 96 hours before collection of the first culture supernatant, and
• the third culture supernatant is a culture supernatant collected from the culture broth at any time from 24 hours to 96 hours after collection of the first culture supernatant.