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US 2025/0145939 A1Published application
Paenibacillus strains producing low amounts of exopolysaccarides
AssigneeBASF SE
InventorsTobias May, Daniel Christoph Heinrich, Andrea Herold, Reinhard Stierl
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Abstract
Disclosed are Paenibacillus strains comprising a reduced activity of a flippase PepR aflippase PepH, a mannose-1-phosphate guanylyl transferase and/or a levansucrase SacB. These Paenibacillus strains show lower viscosity when grown in liquid culture and agricultural compositions comprising these strains and methods of making and using these strains.
Claims14
A Paenibacillus sp. strain comprising
- a) no or a mutant PepR or
- b) a mutant PepH, or
- c) no or a mutant PepX, or
- d) a mutant ManC, or
- e) a mutant SacB, or
- f) a combination of at least two of a), b), c), d) or e),
- wherein
- the missing PepR or the mutant PepR results in a decreased viscosity of a liquid culture of the Paenibacillus sp. strain in comparison to a liquid culture of a Paenibacillus sp. strain comprising a wildtype PepR and
- the mutant PepH results in a decreased viscosity of a liquid culture of the Paenibacillus sp. strain in comparison to a liquid culture of a Paenibacillus sp. strain comprising a wildtype PepH and
- the missing PepX or the mutant PepX results in a decreased viscosity of a liquid culture of the Paenibacillus sp. strain in comparison to a liquid culture of a Paenibacillus sp. strain comprising a wildtype PepX and
- the mutant ManC results in a decreased viscosity of a liquid culture of the Paenibacillus sp. strain in comparison to a liquid culture of a Paenibacillus sp. strain comprising a wildtype ManC or in comparison to a liquid culture of a Paenibacillus sp. strain comprising no ManC and
- the mutant SacB results in a decreased viscosity of a liquid culture of the Paenibacillus sp. strain in comparison to a liquid culture of a Paenibacillus sp. strain comprising no SacB, and
- wherein the Paenibacillus sp. strain comprising the respective wildtype PepR, PepH, PepX, ManC or SacB and used for comparison is of the same Paenibacillus species than the comprising no PepR, PepH, PepX, ManC or SacB or a mutant thereof.
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Dependencies are inferred from the claim text.