Evaporation-limiting inserts for reagent containers and related methods of use

An insert for a liquid-holding container, the insert comprising:

• a body comprising a wall, an open top end, an open bottom end, and a generally tubular lumen extending from the open top end to the open bottom end;
• a plurality of first openings formed in the wall, the first openings being situated between the top and bottom ends, wherein each of the plurality of first openings defines an area, wherein the plurality of first openings have substantially the same dimensions, wherein the plurality of first openings are arranged in a first row of at least three first openings in axial alignment with each other and a second row of at least three first openings in axial alignment with each other, and wherein the first and second rows are situated on opposite sides of the body;
• and
• one or more second openings formed in the wall, the one or more second openings being situated between the top and bottom ends, wherein each of the one or more second openings defines an area that is greater than the area defined by any of the plurality of first openings, wherein each of the one or more second openings have substantially the same dimensions, wherein at least one of the one or more second openings is situated closer to the top end than any of the first openings, and wherein each of the first and second openings is sized to permit the passage of a liquid.