US 2025/0144145 A1Published application

Engineered psd protein, extracellular vesicle, and preparation method and use thereof

AssigneeBeijing Echo Biotech Co., Ltd.

InventorsLibo Zhao, Jiaxun Zhang, Guanyi Kong, Hang Zhao, Da LIU, Zhicheng You

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Abstract

The application discloses an engineered phosphatidylserine decarboxylase (PSD protein) and use thereof, a fusion protein comprising the engineered PSD protein and use thereof, a nucleic acid molecule for encoding the engineered PSD protein or the fusion protein, an expression vector comprising the nucleic acid molecule, an engineered cell comprising the nucleic acid molecule or the expression vector, a method for preparing an engineered extracellular vesicle, an engineered extracellular vesicle and use thereof. In this application, an engineered phosphatidylserine decarboxylase is used for catalyzing phosphatidylserine on the surface of an extracellular vesicle, so that phagocytosis of macrophages is reduced, in-vivo clearance is reduced, and the circulation time of the extracellular vesicle is prolonged.

Claims20

Dependent claims

An engineered phosphatidylserine decarboxylase PSD protein, comprising the following three functional domains:

X1X2X3X4(X5)mX6(X7)nX8X9X10 at positions 114-139, X11X12X13X14X15X16X17X18X19X20 at positions 191-120, and X21X22X23 at positions 307-309, referring to the wild-type PSD protein represented by SEQ ID NO:
1;
wherein X1 and X2 are each independently Phe, Trp, Leu, Val, Ile or Tyr;
X4 and X6 are each independently Arg, Lys, Gln or Asn;
X8, X11, and X20 are Pro;
X10 is Asp, Glu or Asn;
X14 is Tyr, Trp, Phe, Thr or Ser;
X15, X18, and X19 are each independently His, Asn, Gln, Lys, or Arg;
X21 is Gly;
X22 is Ser;
X23 is Ser, Thr or Val;
X3, X5, X7, X9, X12, X13, X16, and X17 are each independently any amino acid, m and n respectively represent m and n repeated amino acids, wherein m=1-6, 2-5, 3-4, or any positive integer within such a numerical range, and n=1-12, 2-11, 3-10, 4-9, 5-8, 6-7, or any positive integer within such a numerical range.

9 more elements

Dependencies are inferred from the claim text.