# US 2025/0144005 A1 · Fermentation extracts, cosmetic composition and manufacturing method of fermentation extracts

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- Number: US 2025/0144005 A1
- Document ID: US20250144005A1
- Status: Published application
- Kind: A1
- Application number: 18/646,804
- Filed: 2024-04-26
- Published: 2025-05-08
- Earliest claimed: 2023-11-02
- Assignees: bioMOA Medical, Inc.
- Applicants: bioMOA Medical, Inc.
- Inventors: Dong-Han Lee
- Web page: https://patentgrep.com/patent/US20250144005A1

## CPC

- A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria
- A61K 8/14: Liposomes; Vesicles
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 8/65: Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
- A61Q 19/00: Preparations for care of the skin
- A61Q 19/02: for chemically bleaching or whitening the skin
- A61Q 19/08: Anti-ageing preparations
- C12N 1/20: Bacteria; Culture media therefor
- A61K 2800/10: General cosmetic use
- A61K 2800/85: Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Provided are fermentation extracts, cosmetic composition, and manufacturing method of fermentation extracts. For this, the present invention discloses a fermentation extract consisting of a glutathione liquid dispersion liposomized using a liquid collagen extract as a solvent and a Lactobacillus fermentation filtrate obtained by stirring and extracting the same at a preset low temperature.

## Claims

### Claim 1

A fermentation extract consisting of a glutathione liquid dispersion liposomized using a liquid collagen extract as a solvent and a Lactobacillus fermentation filtrate obtained by stirring and extracting the same at a preset low temperature.

### Claim 2 (depends on 1)

The fermentation extract according to claim 1, wherein the preset low temperature is 25° C.

### Claim 3 (depends on 1)

The fermentation extract according to claim 1, wherein the liquid collagen extract is extracted at a low temperature of 20° C. by mixing collagen powder, butylene glycol, and purified water at a preset ratio.

### Claim 4 (depends on 1)

The fermentation extract according to claim 1, wherein the liposomized glutathione liquid dispersion is a dispersion obtained by mixing and stirring purified water and glutathione powder at a preset ratio.

### Claim 5 (depends on 1)

The fermentation extract according to claim 1, wherein the Lactobacillus fermentation filtrate is a filtrate obtained by mixing purified water, a Lactobacillus fermentation solution, and glycerin at a preset ratio and fermenting.

### Claim 6 (depends on 1)

A cosmetic composition comprising a fermentation extract of claim 1.

### Claim 7

A manufacturing method of a fermentation extract using dual low-temperature extraction and liposomization, the method comprising:
a step of producing a liquid collagen extract at a first low temperature by mixing collagen powder, butylene glycol, and purified water at a preset ratio;
a step of producing a liposomized glutathione liquid dispersion by mixing and stirring purified water and glutathione powder at a preset ratio;
a step of producing a Lactobacillus fermentation filtrate obtained by mixing purified water, a Lactobacillus fermentation solution, and glycerin at a preset ratio and fermenting; and
a step of producing a fermentation extract by stirring and extracting a glutathione liquid dispersion liposomized using a liquid collagen extract as a solvent and a Lactobacillus fermentation filtrate at a second low temperature which is different from the first low temperature.

### Claim 8 (depends on 7)

The manufacturing method of a fermentation extract according to claim 7, wherein the step of producing a liquid collagen extract includes:
a step of preparing low-molecular-weight collagen powder in a mesh screen of 400 mesh;
a step of setting the temperature of an extractor to 20° C.;
a step of placing a mesh screen containing 30 parts by weight of butylene glycol, 65 parts by weight of purified water, and 5 parts by weight of collagen powder into the extractor;
a step of extracting in the extractor for 250 to 300 hours; and
a step of filtering a completely extracted mixture through the mesh screen of 400 mesh to complete a liquid collagen extract extracted at a low temperature.

### Claim 9 (depends on 7)

The manufacturing method of a fermentation extract according to claim 7, wherein the step of producing a liposomized glutathione liquid dispersion includes:
a step of preparing 90 parts by weight of purified water and 10 parts by weight of glutathione powder;
a step of primarily mixing the purified water with the glutathione in a stirrer for 30 minutes;
a step of producing a homogeneous dispersion by passing the primarily stirred mixed liquid through a ultra-high pressure emulsification process; and
a step of filtering the homogeneous dispersion with a filter screen of 250 mesh to complete a liposomized glutathione liquid dispersion.

### Claim 10 (depends on 7)

The manufacturing method of a fermentation extract according to claim 7, wherein the step of producing a producing a Lactobacillus fermentation filtrate includes:
a step of producing a Lactobacillus fermentation solution by preparing a Lactobacillus strain, inoculating the same into a liquid medium, maintaining the temperature of the liquid medium at 35 to 40° C., and culturing in an incubator for three days;
a step of adding 50 parts by weight of purified water, 30 parts by weight of the Lactobacillus fermentation solution, and 20 parts by weight of glycerin into a fermenter and fermenting at 37° C. for ten hours; and
a step of filtering the completely fermented product through a mesh screen of 400 mesh to complete a Lactobacillus fermentation filtrate.

### Claim 11 (depends on 7)

The manufacturing method of a fermentation extract according to claim 7, wherein the step of producing a fermentation extract includes:
a step of setting the temperature of an extractor to 25° C.;
a step of adding 60 parts by weight of a liquid collagen extract as a solvent, 20 parts by weight of liposomized glutathione liquid dispersion, and 20 parts by weight of the Lactobacillus fermentation filtrate into the extractor and mixing;
a step of extracting for 300 hours while performing a stirring process; and
a step of filtering the completely extract through a mesh screen of 400 mesh to complete a fermentation extract.