Compositions for gut health
AssigneeInternational N&H Denmark Aps
InventorsTanja GRUBER, Ryan FRISCH, Felipe BENDEZU, Gerda SAXER QUANCE
Life of this patent
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Abstract
Provided herein, inter alia, are compositions of organic acid-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.
Claims43
A biologically pure strain of Lactobacillus reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147267.
A biologically pure strain of Lactobacillus reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1b deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147268.
A biologically pure strain of Lactobacillus reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147269.
A biologically pure strain of Lactobacillus reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2b deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147270.
A feed additive composition comprising a direct fed microbial (DFM) comprising one or more biologically pure strains of Lactobacillus reuteri selected from the group consisting of (a) a biologically pure strain of L. reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147267;
- (b) a biologically pure strain of L. reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1b deposited at WFDB under number CBS 147268;
- (c) a biologically pure strain of L. reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2a deposited at WFDB under number CBS 147269;
- and (d) a biologically pure strain of L. reuteri displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2b deposited at WFDB under number CBS 147270.
A bacterial consortium comprising one or more of (a) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147267;
- (b) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1b deposited at WFDB under number CBS 147268;
- (c) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2a deposited at WFDB under number CBS 147269;
- (d) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2b deposited at WFDB under number CBS 147270;
- and/or (e) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S3 deposited at WFDB under number CBS 145923.
The consortium of claim 24, comprising one or more of (a) L. reuteri strain S1a (CBS 147267) or a live strain having all of the identifying characteristics of L. reuteri strain S1a (CBS 147267);
- (b) L. reuteri strain S1b (CBS 147268) or a live strain having all of the identifying characteristics of L. reuteri strain S1b (CBS 147268);
- (c) L. reuteri strain S2a (CBS 147269) or a live strain having all of the identifying characteristics of L. reuteri strain S2a (CBS 147269);
- (d) L. reuteri strain S2b (CBS 147270) or a live strain having all of the identifying characteristics of L. reuteri strain S2b (CBS 147270);
- (e) L. reuteri strain S3 (CBS 145923) or a live strain having all of the identifying characteristics of L. reuteri strain S3 (CBS 145923) either (A) alone;
- or (B) in combination with a culture supernatant derived from one or more of these strains.
A method for preparing a feed additive composition or a bacterial consortium comprising combining one or more of (a) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1a deposited at Westerdijk Fungal Biodiversity Institute (WFDB) under number CBS 147267;
- (b) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S1b deposited at WFDB under number CBS 147268;
- (c) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2a deposited at WFDB under number CBS 147269;
- (d) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S2b deposited at WFDB under number CBS 147270;
- and/or (e) a bacterial strain having a 16S ribosomal RNA sequence displaying at least 97.0% sequence similarity to a 16S ribosomal RNA sequence of a L. reuteri strain S3 deposited at WFDB under number CBS 145923.
The method of claim 49, wherein the composition or consortium comprises one or more of (a) L. reuteri strain S1a (CBS 147267) or a live strain having all of the identifying characteristics of L. reuteri strain S1a (CBS 147267);
- (b) L. reuteri strain S1b (CBS 147268) or a live strain having all of the identifying characteristics of L. reuteri strain S1b (CBS 147268);
- (c) L. reuteri strain S2a (CBS 147269) or a live strain having all of the identifying characteristics of L. reuteri strain S2a (CBS 147269);
- (d) L. reuteri strain S2b (CBS 147270) or a live strain having all of the identifying characteristics of L. reuteri strain S2b (CBS 147270);
- (e) L. reuteri strain S3 (CBS 145923) or a live strain having all of the identifying characteristics of L. reuteri strain S3 (CBS 145923) either (A) alone;
- or (B) in combination with a culture supernatant derived from one or more of these strains.
Dependencies are inferred from the claim text.