Metabolically engineered cells for jasmonic acid biosynthesis
AssigneeSumitomo Chemical Company, Limited
InventorsDavid Nunn, Hui Chen
Life of this patent
Estimating the term…
Abstract
The present invention relates, in some aspects, to a host cell comprising a metabolic pathway producing jasmonic acid. The host cell overexpresses a polypeptide having 12-oxophytodienoate reductase (OPR) activity relative to a corresponding parental host cell.
Claims23
A recombinant host cell comprising a metabolic pathway producing jasmonic acid, wherein the host cell overexpresses a polypeptide having 12-oxophytodienoate reductase (OPR) activity relative to a corresponding parental host cell, wherein the polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO:1.
A recombinant host cell comprising a metabolic pathway producing jasmonic acid, wherein the host cell overexpresses a gene encoding for a polypeptide having 12-oxophytodienoate reductase (OPR) activity relative to a corresponding parental host cell, wherein the gene comprises a polynucleotide sequence having at least 90% identity to the polynucleotide sequence as set forth in SEQ ID NO:2.
16-19. (canceled)
A vector comprising a polynucleotide sequence having at least 90% identity to the polynucleotide sequence as set forth in SEQ ID NO:2.
(canceled)
A recombinant host cell overexpressing a gene encoding for a polypeptide having 12-oxophytodienoate reductase (OPR) activity relative to a corresponding parental host cell, wherein the gene comprises a polynucleotide sequence having at least 90% identity to the polynucleotide sequence as set forth in SEQ ID NO:3.
(canceled)
Dependencies are inferred from the claim text.