Methods for efficient fermention broth recycle
AssigneeSynata Bio, Inc.
InventorsDavid Harvey KEATING, Matthew Theodore KISSINGER
Life of this patent
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Abstract
Disclosed are methods for efficient fermentation broth recycle, methods for improving bottoms recycle, and methods for converting CO, CO2, and optionally H2 to ethanol and other oxygenated products, the methods comprising providing to a bioreactor a gaseous substrate comprising CO, CO2, and optionally H2, at least one acetogenic carboxydotrophic bacterium, and a liquid nutrient medium, and providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert CO, CO2, and optionally H2 to at least one oxygenated product. Also disclosed are methods for preparing animal feed and for preparing fertilizer.
Claims17
A method for efficient fermentation broth recycle, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2;
- (2) at least one acetogenic carboxydotrophic bacterium;
- and (3) a liquid nutrient medium;
- and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert CO, CO2, and optionally H2 to at least one oxygenated product,
- wherein the at least one acetogenic carboxydotrophic bacterium comprises an upstream region sequence for a hydrogenase gene cluster,
- the hydrogenase gene cluster comprises at least a first gene,
- the upstream region sequence is upstream of the first gene,
- the upstream region sequence comprises at least about 90% identity to SEQ ID NO:
- 2, and the conditions within the bioreactor create a bioreactor broth.
A method for improving bottoms recycle, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2;
- (2) at least one acetogenic carboxydotrophic bacterium;
- and (3) a liquid nutrient medium;
- and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert CO, CO2 and H2 to at least one oxygenated product;
- wherein the at least one acetogenic carboxydotrophic bacterium comprises an upstream region sequence for a hydrogenase gene cluster,
- the hydrogenase gene cluster comprises at least a first gene,
- the upstream region sequence is upstream of the first gene,
- the upstream region sequence comprises at least about 90% identity SEQ ID NO:
- 2, and the conditions within the bioreactor create a bioreactor broth.
A method for converting CO, CO2, and optionally H2 to ethanol, the method comprising:
- (a) providing to a bioreactor:
- (1) a gaseous substrate comprising CO, CO2, and optionally H2;
- (2) at least one acetogenic carboxydotrophic bacterium;
- and (3) a liquid nutrient medium;
- and
- (b) providing conditions within the bioreactor for the at least one acetogenic carboxydotrophic bacterium to convert CO, CO2, and optionally H2 to at least one oxygenated product;
- wherein the at least one acetogenic carboxydotrophic bacterium comprises an upstream region sequence for a hydrogenase gene cluster,
- the hydrogenase gene cluster comprises at least a first gene,
- the upstream region sequence is upstream of the first gene,
- the upstream region sequence comprises at least about 90% identity SEQ ID NO:
- 2, and the conditions within the bioreactor create a bioreactor broth.
-18. (canceled)
Dependencies are inferred from the claim text.