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US 2025/0136948 A1Published application
Induction of pluripotent cells
AssigneeThe Scripps Research Institute
InventorsTongxiang Lin, Sheng Ding
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Abstract
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (>200 fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, non-viral methods for reprogramming human somatic cells.
Claims19
A composition comprising:
- (i) a 3′-phosphoinositide-dependent kinase-1 (PDK1) activator;
- (ii) a Rho kinase (ROCK) inhibitor;
- (iii) an ALK5 inhibitor;
- and
- (iv) a MAP/ERK kinase (MEK) inhibitor.
2 more elements
A mixture comprising:
- one or more mammalian cells;
- a PDK1 activator;
- a ROCK inhibitor;
- an ALK5 inhibitor;
- and
- a MEK inhibitor.
3 more elements
Dependencies are inferred from the claim text.