Discrete peg molecules as analytes for ms calibration and tuning in positive and negative modes
AssigneeDH Technologies Development Pte. Ltd.
InventorsSubhasish Purkayastha, Subhakar Dey
Life of this patent
Estimating the term…
Abstract
The disclosure provides compositions, methods, and kits that find use in calibrating a mass spectrometer, and can include one or more predetermined concentration(s) of one or more calibrant molecule(s) that comprise a polyethylene glycol (PEG) compounds that have a single functional group that can be ionized by an ion source, and a solvent for dissolving the calibrant molecule(s). The calibrant molecule(s) and compositions including them can be used in either positive or negative ionization mode, and can be used for calibrating a variety of mass spectrometers (e.g., APCI, ESI) operating in a variety of acquisition modes (e.g., MRM, MS/MS, etc.).
Claims22
(canceled)
(canceled)
A composition comprising a plurality of discrete polyethylene glycol compounds (dPEGs), wherein the plurality of dPEGs comprises a functional group that is ionizable under only one of positive mode ionization or negative mode ionization, wherein the plurality of dPEGs comprise one or more (I) dPEG-amine derivatives, (II) dPEG-carboxylic acid derivatives, (III) dPEG-sulfonic acid derivatives, and/or (IV) dPEG-phenolic derivatives.
(canceled)
A method for calibrating a mass spectrometer comprising:
- a) obtaining a mass spectrum of a composition comprising a plurality of discrete polyethylene glycol compounds (dPEGs), wherein the plurality of dPEGs comprises a functional group that is ionizable under only one of positive mode ionization or negative mode ionization, wherein the plurality of dPEGs comprise one or more (I) dPEG-amine derivatives, (II) dPEG-carboxylic acid derivatives, (III) dPEG-sulfonic acid derivatives, and/or (IV) dPEG-phenolic derivatives;
- b) determining the differences between the expected mass peaks for the plurality of dPEG derivatives in the composition, and the corresponding actual mass peaks in the mass spectrum;
- and
- c) adjusting the mass spectrometer based on the differences between the expected mass peaks and the actual mass peaks.
Dependencies are inferred from the claim text.