Hepatitis c virus e1/e2 heterodimers and methods of producing same
OwnerMichael Houghton, Darren Hockman +3inventors
InventorsMichael Houghton, Darren Hockman, John L. Law, Chao Chen, Michael Logan
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Abstract
The present disclosure provides affinity tagged heterodimeric polypeptides comprising a hepatitis C virus (HCV) E1 polypeptide and an HCV E2 polypeptide, where one or both of the E1 and E2 polypeptides comprises an affinity tag. The present disclosure provides a method of producing an affinity tagged E1/E2 heterodimer of the present disclosure. The present disclosure provides methods of producing untagged HCV E1/E2 heterodimers. The present disclosure provides HCV E1/E2 heterodimers, compositions comprising same, and methods of inducing an immune response to HCV.
Claims21
-80. (canceled)
A nucleic acid comprising a nucleotide sequence encoding a polyprotein comprising, in order from N-terminus to C-terminus:
- a) an HCV E1 polypeptide;
- and
- b) an HCV E2-affinity tag fusion polypeptide comprising, in order from N-terminus to C-terminus:
- i) an affinity tag polypeptide;
- ii) a proteolytically cleavable linker;
- and iii) an HCV E2 polypeptide.
Dependencies are inferred from the claim text.