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AssigneeIntervet Inc.
InventorsLuciana ARAMUNI GONCALVES, Luis Fernando VETTORATO, Francisco BONOMI BARUFI, Helena FACCIOLI LOPES, Mayka RABELO HENRIQUES, Jeane FERREIRA LEAL DE FREITAS, Simone Cristina ROSA
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The present invention relates to the fields of veterinary medicine and virology and provides a vaccine against leishmaniasis. In particular, the invention relates to an isolated polynucleotide comprising (i) a first expression cassette comprising a nucleic acid encoding heat shock protein (hsp) 65, and (ii) a second expression cassette comprising a nucleic acid encoding LACK (Leishmania homologue of receptors for activated C kinase). In other embodiments, the invention relates to a DNA plasmid comprising the polynucleotide, a DNA vaccine for use in the treatment of leishmaniasis, and a method of immunizing a subject against an infection with leishmaniasis.
An isolated polynucleotide comprising:
The polynucleotide of any one of the preceding claims, wherein the nucleic acids encoding hsp65 and LACK are under control of different promoters.
The polynucleotide of any one of the preceding claims, wherein the nucleic acid encoding hsp65 is under control of a cytomegalovirus (CMV) promoter.
The polynucleotide of any one of the preceding claims, wherein the nucleic acid encoding LACK is under the control of an EF1α-HTLV promoter.
The polynucleotide of any one of the preceding claims, wherein the second expression cassette further comprises one or more CpG motifs.
Dependencies are inferred from the claim text.