Novel methods for decellularizing extracellular matrix
AssigneeRegenX Science, Inc.
InventorsRamon B. Montero, Jun-Hung Cho, Elisabeth A. Corson
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Abstract
The present disclosure describes a novel method for decellularizing amphibian extracellular matrix to obtain an isolated ECM that is substantially free of cellular and nuclear debris. The method includes washing the amphibian ECM in solutions containing one or more surfactants, incubating the ECM with one or more proteases, and incubating the ECM with one or more nucleases. The present disclosure also describes various methods of using the isolated ECM.
Claims31
An isolated amphibian ECM, wherein the ECM is decellularized and comprises
- an intact native ECM structure;
- only 1% to 10% or 2% to 10% of the total DNA present in a corresponding native ECM sample;
- residual DNA fragments of less than 300 bases (bps) in length, 50 bps to 300 bps in length, or less than 250 bps in length;
- and/or
- greater than 70% or 80% to 90% of its cells removed.
-5 (canceled)
A method of decellularizing amphibian ECM, wherein the method comprises harvesting a biological sample from an amphibian, washing the sample with a detergent solution, and treating the sample with one or more proteases and one or more nucleases to obtain an isolated ECM.
-32. (canceled)
Dependencies are inferred from the claim text.