Enzyme agent for collagen tripeptide production or cartilage degradation
AssigneeAmano Enzyme Inc.
InventorsMasahiro Kamon, Hiroyuki Yachi, Yuki Ishigaki
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Abstract
It is an object of the present invention to provide a highly safe protease (collagenase) that is useful for food or medical applications, such as collagen tripeptide production or cartilage degradation, the intended use thereof, and the like. According to the present invention, provided is an enzyme agent for collagen tripeptide production, comprising, as an active ingredient, a collagenase consisting of the amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence equivalent thereto.
Claims5
An enzyme agent for collagen tripeptide production, comprising, as an active ingredient, a collagenase consisting of the amino acid sequence as set forth in SEQ ID NO:
- 1 or an amino acid sequence equivalent thereto.
An enzyme agent for collagen tripeptide production, which is derived from Lysinibacillus fusiformis and comprises, as an active ingredient, a collagenase having the following physicochemical properties:
- (1) optimal temperature:
- around 40° C.;
- (2) temperature stability:
- relative residual activity to the activity at the treatment temperature with the highest activity as 100% is 80% or more from 4° C. to 40° C., and is 10% or less at 50° C.;
- (3) optimal pH:
- around pH 7;
- and
- (4) pH stability:
- relative residual activity to the activity at the treatment pH with the highest activity as 100% is 80% or more in the pH range of 6 to 8, and is 10% or less at pH 5.
5-10. (canceled)
Dependencies are inferred from the claim text.