Bacteriophage having bacteriolysis activity on xanthomonas spp
AssigneesKaneka Corporation, Rakuno Gakuen University, Mitsui & Co., Ltd.
InventorsShinichi Yoshida, Nobuhiko Dojun, Mitsuaki Kitano, Hidetomo Iwano, Hitoshi Kudo
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Abstract
To control plant diseases caused by Xanthomonas spp., a novel bacteriophage exhibiting bacteriolysis activity specifically on Xanthomonas spp. was isolated. Thus, developed and provided is a plant disease control composition containing the bacteriophage as an active ingredient. Provided are a bacteriolytic agent containing a bacteriophage that has a novel genomic DNA sequence and exhibits bacteriolysis activity specifically on Xanthomonas spp., and a plant disease control composition containing the same as an active ingredient.
Claims15
A bacteriolytic agent consisting of a bacteriophage comprising, in a genomic DNA, a gene encoding a tail fiber protein having a recognizing ability for target bacteria, wherein the tail fiber protein consists of an amino acid sequence of any one of the following (a) to (d):
- (a) an amino acid sequence of any one selected from the group consisting of SEQ ID NOs:
- 11, 45, 42, and 1;
- (b) an amino acid sequence having addition, deletion, and/or substitution of one or a plurality of amino acids in the amino acid sequence of SEQ ID NO:
- 42 or 1;
- (c) an amino acid sequence having a sequence identity of 90% or more to the amino acid sequence of SEQ ID NO:
- 42 or 1;
- or
- (d) an amino acid sequence having substitution of one amino acid other than at positions 278 and 350 in the amino acid sequence of SEQ ID NO:
- 11.
A bacteriolytic agent consisting of a bacteriophage comprising, in a genomic DNA:
- a gene consisting of an amino acid sequence of any one of the following (e) to (g);
- and
- a gene consisting of an amino acid sequence of any one of the following (h) to (j),
- wherein the genes are respectively a gene encoding a tail tubular protein A and a gene encoding a tail tubular protein B, both protein A and protein B having a recognizing ability for target bacteria:
- (e) an amino acid sequence of SEQ ID NO:
- 24, 49, or 60;
- (f) an amino acid sequence having addition, deletion, and/or substitution of one or a plurality of amino acids in the amino acid sequence of SEQ ID NO:
- 24 or 49;
- (g) an amino acid sequence having a sequence identity of 97% or more to the amino acid sequence of SEQ ID NO:
- 24 or 49;
- (h) an amino acid sequence of SEQ ID NO:
- 57, 50, or 61;
- (i) an amino acid sequence having addition, deletion, and/or substitution of one or a plurality of amino acids in the amino acid sequence of SEQ ID NO:
- 57 or 50;
- or
- (j) an amino acid sequence having a sequence identity of 97% or more to the amino acid sequence of SEQ ID NO:
- 57 or 50.
A bacteriolytic agent consisting of a bacteriophage comprising, in a genomic DNA:
- a gene consisting of an amino acid sequence of any one of the following (k) to (m);
- and
- a gene consisting of an amino acid sequence of any one of the following (n) to (p),
- wherein the genes are respectively a gene encoding a tail tubular protein A and a gene encoding a tail tubular protein B, both protein A and protein B having a recognizing ability for target bacteria:
- (k) an amino acid sequence of SEQ ID NO:
- 37;
- (l) an amino acid sequence having addition, deletion, and/or substitution of one or a plurality of amino acids in the amino acid sequence of SEQ ID NO:
- 37;
- (m) an amino acid sequence having a sequence identity of 92% or more to the amino acid sequence of SEQ ID NO:
- 37;
- (n) an amino acid sequence of SEQ ID NO:
- 38, 54, or 59;
- (o) an amino acid sequence having addition, deletion, and/or substitution of one or a plurality of amino acids in the amino acid sequence of SEQ ID NO:
- 38 or 54;
- or
- (p) an amino acid sequence having a sequence identity of 92% or more to the amino acid sequence of SEQ ID NO:
- 38 or 54.
Dependencies are inferred from the claim text.