Laser Induced Fragmentation for MRM Analysis

Abstract

In one aspect, a method for fragmenting ions in a mass spectrometer is disclosed, which includes introducing a plurality of precursor ions into a collision cell of a mass spectrometer, generating a potential barrier in the collision cell to cause at least a portion of ions in the collision cell to be trapped within a region in proximity of said potential barrier, and applying ultraviolet (UV) radiation to said trapped ions so as to cause fragmentation of at least a portion of any of said precursor ions and fragment ions thereof to generate a plurality of product ions such that a space charge generated in said region in proximity of said potential barrier due to accumulation of ions will impart sufficient kinetic energy to at least a portion of the product ions so as to overcome said potential barrier, thereby exiting said region.

Dependencies are inferred from the claim text.