Viral vectors and packaging cell lines
AssigneeUmoja Biopharma, Inc.
InventorsAndrew SCHARENBERG, Laurie BEITZ
Life of this patent
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Abstract
The disclosure relates generally to nucleic acid vectors and packaging cell lines for in vivo expansion of T-cells. More particularly, the disclosure relates to direct intratumoral injection of a lentiviral vector adapted for transduction and drug-mediated expansion of tumor-infiltrating lymphocytes in vivo.
Claims73
A lentiviral particle for activating and efficiently transducing T cells, comprising a nucleic acid sequence encoding a small-molecule controllable T-cell/NK-cell activation receptor, the nucleic acid sequence operatively linked to a promoter, wherein the T-cell/NK-cell activation receptor is capable of being activated by a small molecule.
A nucleic acid comprising a promoter specific to T-cells, NK cells, or T-cells and NK cells, wherein the sequence of the nucleic acid is at least 90% identical to a sequence selected from the group consisting of SEQ ID NOs:
- 1-4.
A packaging cell line for generating lentiviral particles capable of activating and efficiently transducing T cells, comprising cultured cells capable of packaging a lentivirus vector, wherein the cultured cells are genetically engineered to express a T-cell activation or co-stimulation molecule.
A nucleic acid vector comprising a T-cell and/or NK-cell specific promoter operatively linked to a nucleic acid sequence encoding T-cell/NK-cell activation receptor, wherein the T-cell/NK-cell activation receptor is capable of being activated by a small molecule.
A nucleic acid vector comprising a strong promoter operatively linked to a nucleic acid sequence encoding a T-cell/NK-cell activation receptor, wherein the T-cell/NK-cell activation receptor is capable of being activated by a small molecule.
A nucleic acid vector comprising a sequence at least 70%, 80%, 90%, 95%, or 99% identical to a sequence selected from SEQ ID NOs:
- 6-11.
Dependencies are inferred from the claim text.