Methods and compositions for improving crop yield traits
AssigneesPairwise Plants Services, Inc., Monsanto Technology LLC
InventorsDevin Lee O’Connor, Xiaoyu Zhang, Shunhong Dai, Jennifer Spindel, Thomas Louis Slewinski
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Abstract
This invention relates to compositions and methods for modifying a Adenosine phosphate-isopentenyltransferase 7 (IPT7) gene in plants to improve yield traits. The invention further relates to plants and plant parts produced using the methods and compositions of the invention.
Claims34
A corn plant or plant part thereof comprising at least one mutation in an endogenous Adenosine phosphate-isopentenyltransferase 7 (IPT7) gene encoding an IPT7 polypeptide, wherein the endogenous IPT7 gene:
- (a) comprises a nucleotide sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:69 or SEQ ID NO:70,
- (b) comprises a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:72-107, and/or
- (c) encodes an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:71.
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A method for producing a corn plant or part thereof comprising at least one cell having a mutated endogenous Adenosine phosphate-isopentenyltransferase 7 (IPT7) gene, the method comprising
- contacting a target site within an endogenous IPT7 gene in the corn plant or plant part with a nuclease comprising a cleavage domain and a nucleic acid binding domain, wherein the nucleic acid binding domain of the nuclease binds to a target site within the endogenous IPT7 gene, wherein the endogenous IPT7 gene
- (a) comprises a nucleotide sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:69;
- (b) comprises a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:72-107;
- and/or
- (c) encodes an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:71, thereby producing the corn plant or part thereof comprising at least one cell having a mutation in the endogenous IPT7 gene.
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A guide nucleic acid that binds to a target site within an endogenous gene encoding a Adenosine phosphate-isopentenyltransferase 7 (IPT7) polypeptide, the endogenous gene comprising a nucleotide sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:69, comprising a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:72-73 or 74-92, and/or encoding an amino acid sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:71;
- and/or the target site comprises a nucleotide sequence having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:72-107.
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Dependencies are inferred from the claim text.