T-dna vector encoding a post-translational modification enzyme and lacking regulatory sequences for its expression
AssigneePlantForm Corporation
InventorsMichael D. McLean, John D. Cossar, Wing-Fai Cheung, Haifeng Wang
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Abstract
Plant T-DNA expression vectors encoding a post-translational modification (PTM) enzyme wherein the nucleic acid encoding the PTM enzyme has neither a promoter nor a 5′ untranslated region (UTR) are provided. Also provided are methods of optimizing expression and glycosylation of recombinant protein produced in plants by utilizing the plant T-DNA expression vectors.
Claims17
A plant T-DNA vector comprising a T-DNA region flanked by a Left Border sequence and/or a Right Border sequence, wherein the T-DNA region comprises a nucleic acid molecule encoding a post-translational modification (PTM) enzyme, and wherein
- (a) the ATG start of the translation codon of the nucleic acid sequence encoding the protein of interest is directly adjacent to the Left Border sequence or the Right Border sequence;
- (b) the ATG start of the translation codon of the nucleic acid sequence encoding the protein of interest is within 10, 9, 8, 7, 6, 5 or fewer nucleotides of the Left Border sequence or the Right Border sequence;
- (c) the ATG start of the translation codon of the nucleic acid sequence encoding the protein of interest is directly adjacent to a UTR sequence, and the UTR sequence is directly adjacent to the Left Border sequence or the Right sequence region;
- or
- (d) the ATG start of the translation codon of the nucleic acid sequence encoding the protein of interest is directly adjacent to a UTR sequence, and the UTR sequence is separated by an upstream sequence of 100 base pairs or less from the Left Border sequence or the Right Border sequence.
Dependencies are inferred from the claim text.