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US 2025/0122473 A1Published application
Methods of preparing an isolated or purified population of thymic emigrant cells and methods of treatment using same
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Servic
InventorsRaul E. Vizcardo, Nicholas D. Klemen, Nicholas P. Restifo
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Abstract
Disclosed are methods of preparing thymic emigrant cells in vitro, isolated or purified thymic emigrant cells prepared by the methods, and pharmaceutical compositions comprising the same. Further disclosed are methods of treating or preventing a condition in a mammal comprising administering the thymic emigrant cells or pharmaceutical compositions comprising the same to the mammal.
Claims18
A method of treating or preventing a condition in a mammal, the method comprising:
- modifying source cells into pluripotent cells, multipotent cells, or T-lineage cells;
- culturing the pluripotent cells, multipotent cells, or T-lineage cells in the presence of a Notch receptor agonist to produce CD45+ cells;
- culturing the CD45+ cells in the presence of thymic tissue, wherein culturing the CD45+ cells in the presence of thymic tissue comprises migrating the cells into the thymic tissue;
- egressing the cells from the thymic tissue, wherein the cells egressing from the thymic tissue are thymic emigrant cells;
- isolating the thymic emigrant cells from the thymic tissue, wherein the thymic emigrant cells are CD8α+CD8β+CD4− or CD8α−CD8β−CD4+ and do not express one or more of Ptcra, Rag1, Rag2 and Rorc;
- and
- administering to the mammal the isolated thymic emigrant cells in an amount effective to treat or prevent the condition in the mammal.
4 more elements
Dependencies are inferred from the claim text.