Fluorosurfactants for stabilizing single-and double-emulsion droplets
AssigneeSamplix Aps
InventorsJan SKOUV, Søren MORGENTHALER ECHWALD, Mette JUUL JACOBSEN, Marie JUST MIKKELSEN, Christian HUSSING
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Abstract
The invention provides a fluorosurfactant composition for use in stabilising emulsions, in particular emulsions comprising single or double-emulsion droplets; as well as improved methods for manufacture of the fluorosurfactant; wherein the surfactant comprises a perfluorocarbon chain amide covalently linked to a polymer of ethylene 5 oxide and propylene oxide. The invention also provides methods for single-cell resolution cell killing- and cell secretion-assays based on droplets stabilised by the fluorosurfactant composition.
Claims26
Method of synthesis of a fluorosurfactant having the formula:
- A-X-B or A-X-B-X-A, wherein:
- each instance of A is independently F—[CF(CF3)CF2O]c—CF(CF3)CONH—, wherein c is greater than or equal to 30;
- X, is a covalent bond;
- and
- each instance of B is independently —[C3H6O]d—[C2H4O]e—[C3H6O]fCH2CH(CH3)— or —[C3H6O]g—[C2H4O]h—CH3, wherein a combination of d and f is an integer greater than or equal to 2, e is an integer greater than or equal to 7, each of g and h are integers greater than 0 and a combination of g and h is an integer greater than or equal to 7;
- comprising the synthesis step of reacting the carboxylic end group of a fluoropolymer having the structure F—[CF(CF3)CF2O]cCF(CF3)COOH with oxalyl chloride In the presence of N,N-Dimethylformamide to obtain an activated fluoropolymer intermediate having the structure F—[CF(CF3)CF2O]c—CF(CF3)COCl, wherein the activated fluoropolymer intermediate is subsequently reacted with a mono- or di-functionalized amine polymer of ethylene oxide (EO) and propylene oxide (PO) in a separate step.
Dependencies are inferred from the claim text.