Chimeric virus expressing porcine productive and respiratory syndrome virus-derived peptide and vaccine composition comprising same
AssigneesBiopoa, Inc., Republic of Korea (Animal and Plant Quarantine Agency)
InventorsSang Ho CHA, Seok-Jin KANG, Suhwa YOU, Bang-Hun HYUN, Sun Hee CHO, Changhoon PARK
Life of this patent
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Abstract
Provided are a chimeric virus expressing a porcine reproductive and respiratory syndrome virus (PRRSV)-derived peptide and a use thereof as a vaccine. The chimeric virus has an excellent immune stimulating effect and is useful as a vaccine that can effectively protect against PRRSV by suppressing viral amplification in target cells.
Claims18
A chimeric virus of a PRRSV comprising a polynucleotide consisting of the structure of Structural formula 1 as a genome:
- [Structural formula 1]
- 5′-[X]-[Y]-[Z]-3′
- wherein the [X] is a nucleic acid sequence of NSP1 gene ORF1a and ORF1b regions of LMY virus (GenBank accession no. DQ473474.1) or LMY ver2 virus with accession number KCTC 13394BP, and
- the [Y] is a nucleic acid sequence encoding a polypeptide of SED ID NO:
- 3 or 12, and
- the [Z] is a nucleic acid sequence of ORF2 to ORF7 regions of BP2017-2 virus with accession number KCTC 13393BP.
(canceled)
10-17. (canceled)
A method for producing a chimeric virus of a Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), comprising
- (1) producing a polynucleotide fragment by treating genome of LMY virus (GenBank accession no.DQ473474.1) or LMY ver2 virus of accession number 13394BP with restriction enzymes AscI and PacI;
- (2) preparing a polynucleotide fragment comprising a gene encoding a polynucleotide of SEQ ID NO:
- 3 or 12,
- (3) producing a polynucleotide fragment by treating genome of BP2017-2 virus with accession number KCTC 13393BP with restriction enzymes AscI and PacI;
- and
- (4) producing an infectious clone by recombining the polynucleotide fragments obtained in steps (1) to (3) above.
22-27. (canceled)
A method for prevention or treatment of porcine reproductive and respiratory syndrome, comprising administering
- (1) a chimeric virus of the PRRSV comprising a polynucleotide of Structural formula 2 below as genome or subcultured progeny thereof;
- (2) a chimeric virus of the PRRSV comprising a polynucleotide of Structural formula 3 below as genome or subcultured progeny thereof;
- or
- (3) both of them into a pig:
- [Structural formula 2]
- 5′-[X]-[Ya]-[Z]-3′
- [Structural formula 3]
- 5′-[X]-[Yb]-[Z]-3′
- wherein the [X] is a nucleic acid sequence of NSP1 gene ORF1a and ORF1b regions of LMY virus (GenBank accession no. DQ473474.1) or LMY ver2 virus with accession number KCTC 13394BP, and
- the [Ya] is a nucleic acid sequence comprising an encoding gene of the polypeptide of SED ID NO:
- 3, and
- the [Yb] is a nucleic acid sequence comprising an encoding gene of the polypeptide of SED ID NO:
- 12, and
- the [Z] is a nucleic acid sequence of ORF2 to ORF7 regions of BP2017-2 virus with accession number KCTC 13393BP.
Dependencies are inferred from the claim text.