Cynobacteria cultivation, sedimentation, and utilization
AssigneeThe Texas A&M University System
InventorsShuhua Yuan, Bin Long
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Abstract
The present invention is related to the fields of bioplastics. In particular, a strain of photosynthetic cyanobacteria (i.e. blue-green algae) having a high growth rate was engineered to overexpress limonene synthase. These engineered cyanobacteria improve auto-sedimentation for biomass harvest and provide for plastic bioremediation capture. Further, machine-learning methods are provided for improving some of the aspects of cultivating cyanobacteria including, but not limited to, assessing real-time light availability and predicting real-time algae growth rates to increase a cyanobacteria biomass.
Claims29
A method, comprising:
- a) providing;
- i) a plurality of photosynthetic cyanobacterial cells comprising a transgene comprising a codon optimized plant limonene synthase gene;
- and
- ii) a body of water comprising a plurality of plastic particles;
- b) overexpressing said transgene to display at least one limonene moiety on the surface of said plurality of photosynthetic cyanobacterial cells;
- c) capturing a portion of said plurality of plastic particles with said displayed at least one limonene moiety to create a limonene-plastic particle complex;
- and
- d) sedimenting said limonene-plastic particle complex.
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A composition comprising a photosynthetic cyanobacteria comprising a transgene comprising a codon optimized limonene synthase gene.
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The composition of claim 184, wherein said mint limonene synthase gene is a Mentha spicata limonene synthase gene.
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Dependencies are inferred from the claim text.