Methods Of Producing Nucleic Acids Using Oligonucleotides Modified By A Stimulus
AssigneeTakara Bio USA, Inc.
InventorKazuo Tori
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Abstract
Provided are methods of producing product nucleic acids involving the use of oligonucleotides that are modified by the application of a stimulus. Aspects of such methods may include producing product nucleic acids using de-activatable oligonucleotides that are deactivated by a de-activating stimulus, as well as methods that may include producing product nucleic acids using activatable oligonucleotides that are activated by an activating stimulus and de-activatable oligonucleotides that are deactivated by a de-activating stimulus. Also provided are kits, compositions and devices that include de-activatable oligonucleotides or activatable and de-activatable oligonucleotides, e.g., for use in performing the methods as described herein.
Claims21
-16. (canceled)
A kit comprising:
- a de-activatable template switching oligonucleotide that is deactivated by a first stimulus.
A kit comprising a de-activatable template switching oligonucleotide that is deactivated by a first stimulus and a de-activatable primer that is deactivated by a second stimulus.
Dependencies are inferred from the claim text.