Methods of producing carotenoids from acid whey
AssigneesMassachusetts Institute of Technology, Agricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences
InventorsGregory Stephanopoulos, Yongshou Ma, Konstantinos Katsimpouras, Junichi Mano, Adrian Fay
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Abstract
Disclosed herein are metabolically engineered cells capable of producing carotenoids from acid whey.
Claims37
A genetically modified yeast cell (modified cell) comprising:
- (i) a heterologous gene, wherein the heterologous gene encodes an enzyme having beta-galactosidase (LacA) activity;
- (ii) one or more heterologous genes encoding one or more enzymes capable of converting lactic acid to pyruvate;
- (iii) one or more heterologous genes encoding one or more enzymes of the Leloir pathway;
- and
- (iv) one or more heterologous genes encoding one or more enzymes of the mevalonate pathway.
A genetically modified yeast cell (modified cell) comprising:
- (i) a first heterologous gene, wherein the first heterologous gene encodes an enzyme having beta-carotene ketolase (CrtW) activity;
- and
- (ii) a second heterologous gene, wherein the second heterologous gene encodes an enzyme having beta-carotene hydroxylase (CrtZ) activity;
- wherein the modified cell produces beta-carotene.
An enzyme having lycopene beta cyclase activity comprising an amino acid sequence set forth in SEQ ID NO:
- 2.
An enzyme having lycopene beta cyclase activity comprising an amino acid sequence set forth in SEQ ID NO:
- 3.
An enzyme having lycopene beta cyclase activity comprising an amino acid sequence set forth in SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.