Vector-free intracellular delivery by reversible permeabilisation
AssigneeAvectas Limited
InventorsMichael Maguire, Shirley O'Dea
Life of this patent
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Abstract
The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.
Claims7
A method of delivering a payload across a plasma membrane of a non-adherent cell, comprising,
- providing a population of non-adherent cells;
- and
- contacting the population of cells with a volume of an isotonic aqueous solution, the aqueous solution including the payload and an alcohol at greater than 5 percent (v/v) concentration.
2-20. (canceled)
A system comprising:
- a housing configured to receive a plate comprising a well;
- a differential pressure applicator configured to apply a differential pressure to the well;
- a delivery solution applicator configured to deliver atomized delivery solution to the well;
- a stop solution applicator configured to deliver a stop solution to the well;
- and
- a culture medium applicator configured to deliver a culture medium to the well.
22-54. (canceled)
A composition comprising an isotonic aqueous solution, the aqueous solution comprising KCl at a concentration of 10-500 mM and and ethanol at greater than 5 percent (v/v) concentration for use to deliver a cargo compound or composition to a mammalian cell.
(canceled)
Dependencies are inferred from the claim text.