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US 2025/0101441 A1Published application
Microorganisms and methods for producing cis,cis-muconic acid
AssigneeWisconsin Alumni Research Foundation
InventorsTimothy Donohue, Daniel Noguera, Avery Vilbert, Derek Gille, Wayne S. Kontur
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Abstract
Recombinant microorganisms configured for enhanced production of cis,cis-muconic acid and methods of using the recombinant microorganisms for the production of same.
Claims20
A recombinant microorganism comprising:
- one or more modifications with respect to a corresponding microorganism not comprising the one or more modifications, wherein the one or more modifications comprise at least one of:
- a modification that increases flavin prenyltransferase activity with respect to the corresponding microorganism;
- a modification that increases protocatechuate decarboxylase activity with respect to the corresponding microorganism;
- a recombinant protocatechuate decarboxylase D gene encoding a protocatechuate decarboxylase D protein;
- a modification that increases catechol 1,2-dioxygenase activity with respect to the corresponding microorganism;
- a modification that decreases muconate lactonizing enzyme activity with respect to the corresponding microorganism;
- a modification that decreases muconolactone isomerase activity with respect to the corresponding microorganism;
- a modification that decreases catechol 2,3-dioxygenase activity with respect to the corresponding microorganism;
- and
- a modification that decreases protocatechuate 4,5-dioxygenase activity with respect to the corresponding microorganism.
7 more elements
Dependencies are inferred from the claim text.