Enzyme Variants With Improved Ester Synthase Properties

A variant ester synthase polypeptide comprising an amino acid sequence having at least about 80% identity to SEQ ID NO:

• 67, wherein said variant ester synthase polypeptide is genetically engineered to have at least one mutation at amino acid position corresponding to position 1, 2, 4, 5, 6, 7, 15, 24, 26, 30, 33, 39, 40, 41, 43, 44, 48, 49, 50, 58, 69, 70, 73, 76, 77, 78, 79, 80, 87, 93, 98, 99, 101, 102, 105, 110, 111, 113, 115, 121, 122, 123, 126, 127, 131, 134, 136, 146, 147, 149, 150, 155, 157, 158, 161, 162, 163, 164, 165, 166, 170, 171, 172, 173, 174, 175, 176, 177, 179, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 195, 196, 197, 200, 201, 202, 203, 206, 207, 212, 216, 219, 228, 231, 234, 236, 237, 239, 242, 243, 244, 246, 250, 253, 254, 255, 257, 258, 259, 260, 262, 263, 264, 265, 266, 267, 268, 272, 274, 279, 285, 286, 287, 288, 289, 292, 293, 294, 301, 302, 303, 304, 306, 307, 309, 310, 311, 313, 314, 315, 316, 317, 319, 320, 323, 327, 328, 331, 334, 335, 338, 348, 349, 351, 352, 353, 356, 357, 360, 363, 365, 366, 367, 371, 373, 375, 378, 381, 385, 393, 394, 395, 402, 404, 409, 410, 411, 413, 420, 422, 424, 426, 430, 442, 443, 447, 454, 455, 457, 458, 461, 466, 467, 468, 469, 470, 472, or 474, or a combination thereof, and wherein said variant ester synthase polypeptide has improved ester synthase activity compared to a corresponding wild type polypeptide.